LOCUS       MAN2C1-pGEc2            8346 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(7824..7953)
                     /note="3'-ITR"
     misc_feature    complement(7522..7736)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    6974..7515
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    6153..6175
                     /note="attB2"
     misc_feature    6953..6958
                     /note="BstB1 site"
     misc_feature    6176..6871
                     /note="Super GFP"
     misc_feature    6872..6922
                     /note="AviTag"
     misc_feature    6923..6946
                     /note="His Tag"
     PCR_frame       3011..6152
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    3011..6130
                     /note="OPTIMIZED MAN2C1 coding region"
     misc_feature    6131..6152
                     /note="TEV"
     vector          join(6161..8346,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..6160
                     /source="MAN2C1 pDONR"
                     /type="Entry Clone"
     source          1..8346
                     /dnas_title="MAN2C1-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGGCTGCTG CCCCTGCTCT GAAACATTGG CGGACAACCC TGGAACGGGT
     3061 GGAAAAGTTC GTGTCCCCCC TGTACTTCAC CGACTGCAAC CTGAGAGGCA GACTGTTTGG
     3121 CGCCTCTTGT CCTGTGGCCG TGCTGAGCAG TTTTCTGACC CCTGAGAGAC TGCCCTACCA
     3181 GGAAGCCGTC CAGAGAGATT TCAGACCCGC CCAGGTCGGA GATAGCTTTG GCCCTACATG
     3241 GTGGACCTGC TGGTTCAGAG TGGAACTGAC AATCCCTGAG GCCTGGGTCG GACAGGAAGT
     3301 GCACCTCTGT TGGGAGTCTG ATGGCGAAGG ACTCGTGTGG CGAGATGGGG AACCTGTGCA
     3361 GGGACTGACC AAAGAGGGCG AGAAAACCAG CTACGTGCTG ACCGATAGAC TGGGCGAGAG
     3421 AGATCCTAGA AGCCTGACCC TGTACGTGGA AGTGGCCTGT AATGGACTGC TGGGAGCCGG
     3481 CAAGGGATCT ATGATTGCCG CCCCTGATCC CGAGAAGATG TTCCAGCTGT CTAGAGCCGA
     3541 GCTGGCCGTG TTCCACAGAG ATGTGCATAT GCTGCTGGTG GATCTGGAAC TGCTGCTCGG
     3601 AATTGCCAAG GGCCTGGGCA AGGACAACCA GAGAAGCTTT CAGGCCCTGT ACACCGCCAA
     3661 CCAGATGGTC AACGTGTGTG ACCCTGCCCA GCCTGAGACA TTTCCAGTGG CTCAGGCTCT
     3721 GGCCAGCAGA TTTTTTGGAC AGCATGGCGG CGAGAGCCAG CACACAATCC ATGCTACAGG
     3781 ACACTGCCAC ATCGACACCG CCTGGCTGTG GCCTTTTAAA GAAACCGTGC GGAAGTGCGC
     3841 CAGAAGCTGG GTCACAGCTC TGCAGCTGAT GGAACGGAAC CCCGAGTTCA TCTTTGCCTG
     3901 TAGCCAGGCC CAGCAGCTCG AGTGGGTCAA GTCTAGATAC CCCGGCCTGT ACAGCAGAAT
     3961 CCAGGAATTC GCCTGCAGAG GCCAGTTTGT GCCTGTGGGA GGAACATGGG TCGAGATGGA
     4021 TGGCAATCTG CCTAGCGGAG AAGCTATGGT CCGACAGTTC CTCCAGGGCC AGAACTTCTT
     4081 TCTGCAGGAA TTTGGCAAGA TGTGCTCCGA GTTTTGGCTG CCCGATACCT TTGGCTATAG
     4141 CGCCCAGCTG CCTCAGATCA TGCACGGCTG CGGCATCAGA AGATTTCTGA CACAGAAACT
     4201 GTCCTGGAAC CTGGTCAACA GCTTCCCCCA CCACACATTC TTTTGGGAGG GACTGGATGG
     4261 CTCCCGGGTG CTGGTGCATT TCCCACCTGG CGATTCTTAC GGCATGCAGG GAAGTGTGGA
     4321 AGAGGTGCTG AAAACCGTGG CCAACAACAG AGACAAGGGC AGGGCCAATC ACAGCGCCTT
     4381 TCTGTTCGGA TTTGGAGATG GCGGAGGCGG CCCAACCCAG ACAATGCTGG ATAGGCTGAA
     4441 GCGGCTGAGC AACACCGATG GACTCCCTAG AGTGCAGCTG TCAAGCCCTC GGCAGCTGTT
     4501 CTCTGCCCTG GAATCTGATA GCGAGCAGCT GTGTACTTGG GTCGGCGAAC TGTTCCTCGA
     4561 ACTGCACAAT GGCACCTACA CCACCCACGC CCAGATCAAG AAAGGCAATA GAGAGTGCGA
     4621 GCGGATCCTG CACGATGTGG AACTCCTCAG TAGCCTGGCA CTGGCTAGAT CCGCCCAGTT
     4681 CCTGTATCCT GCAGCTCAGC TCCAGCATCT GTGGCGGCTG CTCCTCCTGA ATCAGTTTCA
     4741 CGATGTCGTG ACCGGCTCCT GCATTCAGAT GGTGGCCGAA GAGGCCATGT GCCACTACGA
     4801 GGATATCAGA AGCCACGGCA ATACCCTGCT GTCTGCCGCT GCTGCTGCAC TGTGTGCCGG
     4861 CGAACCTGGA CCTGAAGGCC TGCTGATTGT GAACACCCTG CCTTGGAAGC GGATCGAAGT
     4921 GATGGCTCTG CCTAAACCTG GCGGAGCCCA TTCTCTGGCT CTGGTCACAG TGCCTTCTAT
     4981 GGGCTATGCT CCTGTGCCTC CTCCAACAAG TCTGCAGCCT CTGCTGCCAC AGCAGCCTGT
     5041 GTTTGTGGTG CAGGAAACAG ACGGCAGCGT CACCCTGGAC AACGGCATCA TCAGAGTGAA
     5101 GCTGGATCCT ACCGGCAGGC TGACATCACT GGTCCTGGTC GCAAGCGGAA GAGAAGCCAT
     5161 TGCTGAAGGC GCCGTGGGCA ACCAGTTCGT GCTGTTTGAT GACGTGCCAC TGTACTGGGA
     5221 CGCCTGGGAC GTGATGGATT ACCACCTGGA AACCAGAAAG CCCGTGCTGG GACAGGCAGG
     5281 CACACTGGCA GTGGGAACAG AAGGTGGACT GAGAGGATCT GCCTGGTTCC TGCTGCAGAT
     5341 CAGCCCCAAT AGCAGACTGA GCCAGGAGGT CGTCCTGGAC GTCGGATGTC CTTACGTGCG
     5401 GTTCCACACC GAAGTGCATT GGCACGAGGC CCACAAGTTT CTGAAGGTCG AGTTTCCAGC
     5461 CAGAGTGCGG TCCTCCCAGG CCACCTATGA GATCCAGTTT GGACACCTCC AGCGCCCCAC
     5521 CCACTACAAC ACAAGTTGGG ACTGGGCCAG ATTTGAAGTC TGGGCTCACC GGTGGATGGA
     5581 TCTGTCTGAG CATGGCTTTG GACTCGCCCT GCTGAACGAT TGCAAATATG GCGCTAGCGT
     5641 GCGGGGCTCC ATTCTGAGTC TGTCTCTGCT GAGAGCCCCT AAGGCTCCTG ATGCTACTGC
     5701 CGATACAGGC AGACACGAGT TCACCTACGC CCTGATGCCT CACAAAGGCA GCTTCCAGGA
     5761 TGCTGGCGTG ATCCAGGCCG CCTACAGCCT GAATTTTCCA CTGCTGGCAC TCCCTGCTCC
     5821 TAGCCCAGCT CCTGCTACAT CTTGGAGCGC TTTTAGCGTG TCCTCTCCCG CCGTGGTGCT
     5881 CGAAACAGTG AAACAGGCCG AAAGCAGCCC TCAGAGAAGA TCCCTGGTGC TGAGACTGTA
     5941 CGAAGCCCAC GGATCTCACG TGGACTGTTG GCTGCATCTG AGCCTGCCAG TCCAGGAGGC
     6001 TATTCTCTGC GATCTGCTCG AGAGGCCTGA TCCTGCTGGA CATCTGACAC TGCGGGACAA
     6061 TAGACTGAAG CTGACATTCA GCCCCTTTCA GGTGCTGTCC CTCCTGCTCG TGCTGCAGCC
     6121 ACCTCCTCAT gaaaacttgt actttcaagg ctCCAGCTTT CTTGTACAAA GTGGTATGAG
     6181 CAAGGGCGAG GAACTGTTCA CCGGCGTGGT GCCCATCCTG GTGGAACTGG ATGGCGACGT
     6241 GAACGGCCAC AAGTTCTCTG TGCGGGGAGA GGGCGAAGGC GACGCCACAA ATGGCAAGCT
     6301 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCTTGGCCTA CCCTCGTGAC
     6361 CACACTGACC TACGGCGTGC AGTGCTTCAG CAGATACCCC GACCATATGA AGCGGCACGA
     6421 CTTCTTCAAG AGCGCCATGC CCGAGGGCTA CGTGCAGGAA CGGACCATCA GCTTCAAGGA
     6481 CGACGGCACC TACAAGACCA GAGCCGAAGT GAAGTTCGAG GGCGACACCC TCGTGAACCG
     6541 GATCGAGCTG AAGGGCATCG ATTTCAAAGA GGACGGCAAC ATCCTGGGCC ACAAGCTGGA
     6601 GTACAACTTC AACAGCCACA ACGTGTACAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA
     6661 GGCCAACTTC AAGATCCGGC ACAACGTGGA AGATGGCAGC GTGCAGCTGG CCGACCACTA
     6721 CCAGCAGAAC ACCCCCATCG GAGATGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
     6781 CACCCAGAGC GTGCTGAGCA AGGACCCCAA CGAGAAGCGG GACCACATGG TGCTGCTGGA
     6841 ATTCGTGACC GCCGCTGGCA TCACCCACGG CATGAGCGGC CTGAACGACA TCTTCGAGGC
     6901 CCAGAAAATC GAGTGGCACG AGCACCACCA TCATCACCAT CACCACTGAT GATTCGAAAA
     6961 ATAATAAGGA TCCAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT
     7021 TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC
     7081 TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT
     7141 TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA
     7201 CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC
     7261 TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC
     7321 AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC TGACGTCCTT
     7381 TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT
     7441 CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC
     7501 TCTTCCGCGT CTTCGAGATC TGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT
     7561 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA
     7621 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG
     7681 GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGACTCG
     7741 AGTTAAGGGC GAATTGCCGA TTAGGATCTT CCTAGAGCAT GGCTACGTAG ATAAGTAGCA
     7801 TGGCGGGTTA ATCATTAACT ACAAGGAACC CCTAGTGATG GAGTTGGCCA CTCCCTCTCT
     7861 GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC CGGGCTTTGC
     7921 CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCCTTAAT TAAGAGCTCA TGGCGCGCCT
     7981 AGGCCTTGAC GGCCTTCCGC CAATTCGCCC TATAGTGAGT CGTATTACGT CGCGCTCACT
     8041 GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT
     8101 TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGAAACGCC
     8161 CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGGAGCG CCCTGTAGCG GCCACTCAAC
     8221 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA
     8281 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA
     8341 ATTTAG
//

